Keratitis is second most common cause of blindness. Prompt treatment has to be started to prevent ocular morbidity. This study was conducted to observe antibacterial susceptibility pattern in Keratitis with regard to Methicillin, Fluoroquinolones and Aminoglycosides. 150 clinically suspected cases of Bacterial keratitis were taken up for the study. Corneal scrapings were inoculated on Blood Agar, Chocolate Agar and MacConkey Agar. Antibiotic susceptibility testing was done by Kirby–Bauer disc diffusion method. Methicillin resistance was tested by Cefoxitin disc diffusion test. Fluoroquinolones used were Ciprofloxacin, Ofloxacin, Gatifloxacin, Moxifloxacin. Aminoglycosides used were Gentamycin and Tobramycin. Total number of culture positive bacterial isolates was 50. Among the culture positive isolates, 96% were gram positive organisms. 27.6% Methicillin resistant Staphylococci were isolated. Among the Fluoroquinolones used, highest resistance of 54% was observed for Ofloxacin. 14% resistance was observed for Tobramycin. Staphylococcus epidermidis is a common isolate in keratitis. Fluoroquinolones and Aminoglycosides are most widely used for the treatment of Bacterial keratitis. However, there is an increase in antibacterial resistance towards the above group of antibiotics.