Background: Vitamin D deficiency is pandemic, yet it is the most under–diagnosed and under–treated nutritional deficiency in the world. It is a serious public health problem, particularly in the Indian sub–continent. Aims and objective: This study was done to evaluate the prevalence of vitamin D deficiency in apparently healthy individuals. Materials and methods: The data of 25–hydroxy(OH) vitamin D assay of 540 healthy individuals, enrolled under executive health checkup at Guwahati Neurology Research Centre and hospital, Guwahati, over a period of two years, were extracted from the hospital information system and was reviewed. 25(OH) vitamin D deficiency was defined as 25(OH) vitamin D< 20 ng/ml, insufficiency as 25(OH) vitamin D between 20 and 29 ng/ml and 25(OH) vitamin D sufficiency (VDS) as 30–100 ng/ml, Potential toxicity>100 ng/ml. Results: A total of 540 healthy individuals were included in this study. There were 300 females and 240 males.365 healthy adults were found to be Vitamin D deficient. Prevelance of Vitamin D deficiency was found to be 67.5%. 225 females and 140 males were found to be Vitamin D deficient, prevalence being 75% in female and 58.3% in male. Conclusion: Our study demonstrates a high prevalence of vitamin D deficiency in apparently healthy individuals.