Stability of serum and plasma glucose was assessed for one year duration of storage at different temperatures. Eighty aliquots each of 150 µl were made from pooled serum and plasma. Fresh sample was analyzed within 4 hours of collection, considered as baseline value. Aliquots of samples were stored at –20˚C & –80˚C and were analyzed over a period of one year and values compared against the baseline. All values of the relative percentage differences for serum and plasma glucose, from its values in fresh samples were within acceptable variations of less than 10%. Our study indicates clinically acceptable stability for Plasma Glucose and Serum Glucose over the period of one year of observation, with better stability in case of Serum Glucose at –80°C.