Introduction : Pseudomonas  aeruginosa  is one of the most common  pathogen causing nosocomial  infections. Emergence of Metallo–betalactamase producing P.aeruginosa (MBL –PA) in hospital  is alarming and poses not only a therapeutic problem, but also a serious concern for infection control management. Hence the present study was conducted to find out the prevalence of MBL–PA isolated from clinical specimens in a tertiary care hospital from Weastern Maharashtra.

Material and methods : A total of 48 strains of P. aeruginosa were recovered from various clinical specimens and screened for imipenem resistance by Kirby Bauer disk diffusion method and imipenem–EDTA combined disk test according to CLSI guidelines.

Results : We found  18.75% MBL–PA ; with highest incidence in surgery, burns unit and ICU specially in high risk patients.

Conclusion : We found high prevalence of MBL–PA  which appears to be worrisome. Rapid detection of MBL producing isolates is necessary to modify therapy and to initiate effective infection control to prevent their dissemination.