Laundromat soil consist many thermostable alkaline protease–producing microorganisms, hence screened for the isolation, identification, and production of thermostable alkaline protease. No such promising production medium defined for the production of alkaline protease, as a different level of enzyme production found with different medium constitutes. Therefore, we reviewed various cultural and media parameters to establish cost–efficient production medium for the strain APP–07.
In conclusion, we established the most suitable production medium for the newly isolated Bacillus licheniformis–APP–07 containing 1.0 % glucose, 0.5% yeast extract, 0.5% peptone, 5.0% NaCl, 0.02% MgSO₄, 0.1% K₂HPO₄, 0.02% CaCl₂, and 1.0% Na₂CO_3, 0.5 % defatted soy flour and 1.0% maltdextrin. The 774.969 U/ml of proteolytic activity was achieved after 33 h cultivation on a rotary shaker (100 rpm) at pH 10.5 and temperature 55⁰C. The enzyme may serve potential applications in detergents, tannery, food, pharmaceutical, diagnostics, protein biochemistry and many other industrial processes.