Introduction: Vitamin D deficiency is common now a days due to increased indoor activity. Vitamin D is a steroid molecule that is mainly produced in the skin and regulates the expression of many genes.Vitamin D has also been shown to influence thyrocytes directly by attenuating TSH. Thyroid hormones influences vitamin D levels.

Objectives– To assess serum 25–OH vitamin D levels in  female hypothyroid patients and compare it with euthyroid females.

Materials and  Methods– 50 patient samples and 50 control samples were taken. Serum was separated and serum25–OH vitamin D levels and thyroid estimation were done on ABOTT ARCHITCT  i1000SR IMMUNOASSAY machine . Data was analyzed using SPSS 20 software.

Results– Cases have significantly lower values for serum 25–OH vitamin D than controls and have significantly negative correlation with TSH.

Conclusions– Hypothyroidism is associated with decreased 25–OH vitamin D .