Today diabetes became a global public health issue. More than 1200 species of plants are identified as herbal remedy for diabetes. In present study, Ethyl acetate and aqueous extract of Acanthospermum hispidum DC. was tested on blood glucose levels (mg/dl) at different time intervals of diabetic induced rats and found that the blood glucose levels are decreased and also able to alter the levels of lipid metabolites including TC,TG, HDL and LDL cholesterol levels in diabetic rats after 9 hours in all the three groups of rats with body weights of 150mg/kg, 300mg/kg and 450mg/kg that are taken for testing.