Blood stream infections range from mild infections to life threatening sepsis requiring appropriate and aggressive anti microbial therapy. A retrospective study was undertaken to identify the microbial profile with their antibiogram among the blood culture isolates in a teaching hospital from June 2017 to November 2017.1101 blood culture samples processed at the microbiology lab were analyzed. The bacterial isolates from positive culture were identified by standard protocols and antimicrobial susceptibility patterns were identified by CLSI guidelines. Positive blood cultures were obtained in 13.7% (151/1101). Gram positive bacteria accounted for 58.27 % (88) and Gram negative 41.72 %( 63) with predominance of S.aureus 39.73% (60) followed by K.pneumoniae 29.8% (45). 18.33% of S.aureus was MRSA and 100% sensitive to vancomycin. 22.44% of K.pneumoniae was ESBL producers. All Gram negative isolates were sensitive to imipenem and meropenem. The present study provides the necessary information on the microbial profile with their antibiogram of the blood stream infections that may help in the formulation of antibiotic policy for effective patient care.