Background: In today’s modern scenario, stress is a part of day to day life. Stress gives rise to various diseases e.g. diabetes mellitus, hypertension, psychosomatic diseases. In diabetes mellitus (T2DM) reaction time, which is a reliable indicator of attention and fine motor skills, was found to be slowed. Hence ways to relieve stress are gaining importance. The Meditation is considered as an antidote to minds vulnerability to toxic emotions and stress. Raja Yoga meditation is simple, and easy to practice method of meditation. The present study is done to find out effect of Raja yoga meditation on auditory reaction time and blood glucose level in T2DM patients. Material and Methods: Study was conducted on three groups, Group I: 30 Non diabetic, non meditators, Group II: 30 Non–meditators, diagnosed T2DM for more than 5 yrs, Group III: 30 diagnosed patients of T2DM for more than 5 yrs and practicing Raja yoga meditation for more than 5 yrs. Their blood glucose level and Auditory Reaction Time (ART) was recorded. Results: The mean value of the ART for High frequency sound (HFS) and Low frequency sound (LFS) were significantly high in diabetic non–meditators as compared to controls (p<0.05) and significantly low in diabetic meditators as compared to diabetic non–meditators. Blood glucose level and glycated hemoglobin levels were significantly lower in diabetic meditators. Conclusion: Practice of Raja yoga meditation may have extensive positive effects on many factors important in T2DM management and prevention, including glycaemic control and ART.