Background Platelets transfusion is an established clinical procedure in thrombocytopenia. The productivity and quality of collection of apheresis platelets have now improved with the new technical advances in automatic cell separators. It is also important to assess the effect of plateletpheresis on donors. Material and Methods In this study pre and post donation haematological parameters namely Complete Blood Count (CBC) and Mean Platelets Volume (MPV) of 100 healthy plateletpheresis donors were recorded and the effects of plateletpheresis procedure on the donor during and after donation were studied. Results Pre and Post donation haematological parameter like Haemoglobin (Hb), Hematocrit(Hct), Platelet count(PC) and White Blood Cell (WBC) count showed statistically significant differences. Conclusion Training modules for the technical personnel, supervision of transfusion medicine specialists, close monitor and follow up of these donors is required.