In relation to the oral manifestations of Down’s syndrome an autosomal disorder, some authors have reported a relationship between low prevalence of dental caries and high prevalence of periodontal disease compared to healthy children. Aim of this study was to evaluate & compare salivary Total Proteins and few electrolytes: Sodium, Potassium, Chloride, Calcium, Zinc in children with Down’s syndrome and healthy children of mixed dentition age group. For this study, detailed clinical examination and un–stimulated whole saliva specimens were collected from 20 children with Down’s syndrome –Group A & healthy children –Group B. The samples were studied for estimation of total proteins and electrolytes content by mass spectrometry and plasma emission spectroscopy. Unpaired t–test was carried out. Concentration of salivary TP, Na, K, Cl, & Ca was higher in Group A when compared with Group B which was converse with the concentration of Zn. On examination low dental caries and higher prevalence of periodontal disease was observed in Group A when compared to Group B. Also, the concentration of TP and Na, K, Cl & Ca was higher in Group A and concentration of Zn was higher in Group B suggesting probable correlation between the oral health.