Background– This study was undertaken to know the multi drug resistance in isolates of Pseudomonas aeruginosa and exploring its relationship with type of specimen, age and gender.

Materials & Methods– 200 isolates of Pseudomonas aeruginosa were studied. The colonies were tested for biochemical tests. Identification was done and then put to Antibiotic susceptibility test by Kirby–Bauer method.

Results & Analysis–. In our study, 63 out of 200 isolates were found to be Multi drug Resistant (31.50 %). 6 MDR isolates out of 10 specimens (60%) detected from burn tissue. 24 out of 60 isolates (40%) were found in Urine,17 out of 75(22.66%)in pus, the lowest 2 out of 9 (22.22%) isolates from blood specimens. 50 out of 63(79.36%) MDR isolates were found in male and only 13out of 63(20.64%) were found in female patients. 21out of 62 (33.87%) MDR isolates were found from ICU followed by 16 out of 34(47.05%) from Surgery dept. The lowest 1 out of 11(9.09%) isolate was from OBG dept. The maximum 18 out of 50 (36%)MDR recorded in the patients between 41 to 60 years age, the lowest 5 out of 25 (20 %)in the age group up to 20 years detected. Highest resistance 36.5%was observed with ceftazidime and piperacillin antibiotics .Polymyxin B, recorded the lowest 3.5% resistance in the study.

Discussion–Pseudomonas aeruginosa are inherently resistant to many antimicrobial agents owing to various resistance mechanisms .63 out of 200 isolates( 31.50%) were found to be resistant to three or more group of antibiotics and thus poses a serious threat to patients and clinicians in their management.

Conclusion–Multi drug resistance were found more prevalent in Male patients in comparison to female. The age between 41– 60 years were worst affected and age group between 0– 20 years were found least affected for MDR. ICU dept. recorded the highest number of MDR isolates where as Gynecology dept. shown least MDR in the study. Burn tissue had shown maximum percentage of MDR