Introduction: Rotavirus has been recognized as most common cause of diarrhoea in children. Objectives: To study prevalence of rotavirus infection in acute diarrhoea among children below five years age and clinical profile of rotaviral diarrhoea. Material and methods:  Present study was conducted at Agartala Government Medical College from Nov–2014 to April–2016. Stool samples from 460 hospitalized children with acute diarrhoea were tested for rotaviral antigen. Caregivers were interviewed, physical examination conducted and assessment of dehydration was done. Results: Out of 460 stool samples, 36% were rotavirus positive. 60.24% cases were below 12 months age group, 61.44% were male children. 66.26% children had fever and 94% cases had vomiting. Mean duration of diarrhoea (4.97 days) and mean duration of hospital stay (3.71 days) was significantly high in Rotaviral diarrhoea as compare with non–rotaviral diarrhoea. Conclusion: Rotavirus is a significant cause of acute diarrhoea and mainly affects children below 12 months age.