Aims:

1.      To stain the known keratin containing tissue by Haematoxylin–Eosin stain,Giemsa stain and Grams stain.

2.      To compare efficacy of Giemsa,Grams and Haematoxylin–Eosin staining technique for demonstration of Keratin.

Objective:

 To device a keratin demonstration procedure which is easy, effective, comparable and advantageous to H–E staining.

Materials and methods:

A total 30 paraffin embedded tissue sections of known keratin containing tissue including hyperkeratotic lesions,well differentiated squamous cell carcinoma and odontogenic keratocyst will be taken.

Results:

 In our study all the three staining showed clear, distinct keratin as pink, Light Pink and Blue respectively.All three stains showed similar results for staining surface  keratin in Hyperkeratotic lesions and OKC .

 Conclusion:

Gram’s and Giemsa staining of keratin in Hyperkeratotic lesions and OKC is comparable to staining with H & E. Overall staining efficacy of keratin by H & E in WDSCC is excellent as compared to Grams, Giemsa.