A total of 122 non fermenting gram negative bacilli isolated from various clinical samples were selected for the study. Isolates which were resistant to third generation cephalosporins selected and tested for ESBL and Amp C production. All the isolates were also tested for biofilm production by tube adherence method. Analyse both to check whether any correlation between these two parameters. Of total 122 isolates of nonfermentors studied Pseudomonas aeruginosa (58.3%) was the commonly isolated and 30% were biofilm producers. Among 122 isolates, 14.8% were ESBL producers and 52.5% were AmpC producers. Isolates producing both ESBL and Biofilm were exhibiting more resistance against common classes of antibiotics than the isolates producing ESBL alone, which proves that there is a significant correlation between ESBL production and biofilm production by Non fermenters.