The aim of study was to evaluate and compare serum Irisin and Gamma Glutamyl Transferase (GGT) activity in type 2 diabetic patient (T2D) and in normal individual. In this case control study, age and sex matched 100 diagnosed cases of type 2 diabetes mellitus were compared with 100 healthy participants at Jawaharlal Nehru Medical College & Associated Group of Hospitals, Ajmer (Rajasthan). Levels of GGT were significantly higher (28.8 ± 8.0 vs 48.0 ± 10.7 IU/Lt.) and levels of Irisin were lower (214.9 ± 28.4 vs 202.2 ± 10.5 ng/ml) in T2D patients (P value < 0.001). GGT was found to be negatively correlated with Irisin (r = –0.946) in T2D patients and positively correlated with Irisin (r = 0.810) in healthy controls. In our study we found myokine Irisin level decreasing gradually with the progression of T2D and increase in GGT level in diabetics in response to oxidative stress.