Blood glucose levels of patient are an important concern for the successful periodontal therapy and stabilization of blood glucose to a near normal range is very important consideration for surgical as well as non– surgical therapy. Sixty patients in the age group of 35–65 years of either sex with type II diabetes mellitus and periodontal disease were selected. Venous blood was used as a control group, whereas finger– prick blood and gingival crevicular blood constituted the study groups I and II respectively. Blood samples were tested in glucometer to check the blood glucose level. The values obtained by glucometer exhibited significant correlations among each other, but the blood glucose values obtained using venous bloodsamples were higher than that of finger prick blood samples followed by gingival blood samples. Hence gingiva can be used as an alternative site to check blood glucose and gingival wound healing is definitely better than finger prick healing.