Introduction: Hybrid rotary instrumentation, involving Tri Auto Mini with Root Zx Mini,  in combination with newer rotary systems, are claimed to provide accurate and efficient length determination along with a centred preparation, retaining the original canal curvature. Methodology: 90 mandibular first molars were selected and prepared on the bases of predetermined criteria with Hyflex CM, Twisted files and Revo S system. Root canal preparation time, working length control, instrument failure and centricity of the prepared canals were evaluated. Collected data was statistically analysed with Shapiro–Wilk test, Kruskal–Wallis, One–way ANOVA and Tukey post hoc test. Result: Hybrid instrumentation successfully maintained the working length in all the samples. Hyflex CM and Twisted Files systems retained the original canal curvature. Preparation was fastest with Twisted files followed by Hyflex CM and Revo S, respectively. Hyflex CM displayed the maximum defects.