We aimed to compare the effects of amifostine (AMI) and L–carnitine (LC) in prevention of radiation induced cardiac injury. Sixty–four rats were divided into 6 groups: Control (CONT), radiotherapy (RT), AMI+RT, LC+RT, and LC. The animals in RT groups received 15 Gy/fraction by 6 MV photon, to the cardiac area. After 6 months follow–up, electrocardiograpy and histopatologic evaluation were carried out. Significant difference was found between AMI+RT and RT, LC+RT and RT, and LC+RT and CONT in terms of myocardial degeneration. Atrial fibrosis was found to be significantly different between AMI+RT and RT. Significant difference was found between groups in terms of ventricular fibrosis. LC and AMI obviously prevented the prolongation of JTa, JTac and QTc intervals after irradiation. The cardioprotective effect of AMI was superior to LC in terms of myocardial degeneration, atrial fibrosis and ventricular fibrosis.