Introduction: Knwoledge of root canal anatomy is of utmost importance as success depends on thorough knowledge of tooth morphology with its complex internal anatomy, thorough shaping and cleaning, and complete fluid tight seal of the root canal system. Objective: To study the root canal morphology of mandibular first molar using CBCT and de Pablos classification. Methods: Total of 90 teeth were scanned using Pro Max 3D CBCT machine followed by image study using Planmeca Romexis software. Results: In lower 1^st molars with 2 roots, the most common canal configuration was type IV (46.7%), followed by type II (30%). Conclusion: This study supported the use of CBCT machine as a potential non–destructive means to study root canal anatomy.