Introduction: Tooth hypersensitivity, or more precisely dentine sensitivity or hypersensitivity is described clinically as an exaggerated response to non–noxious stimuli and satisfies all the criteria to be classified as a true pain syndrome.Aim:To evaluate the occluding effect of three self–applied commercially available dentifrices containing Novamin, dentifrices containing Biomin–F and dentifrices containing potassium nitrate and spinach under scanning electron microscope (SEM).Design:In this study, 10 sound extracted third molars were selected for all three groups and sectioned.Out of all the sections, 30 specimens were taken.The specimens were then divided into three groups with 10 specimens in each group, which were Group 1 – Novamin, Group 2–  Biomin–F, Group 3 – Hiora–k (contains spinach and potassium nitrate)respectively. In all three groups, dentifrice were applied to the specimens with the help of a micro brush, left for 2 minutes and then lightly rinsed away with distilled water, they were mounted on SEM stub. Data obtained was analysed using ANOVA test followed by tukey’s post hoc analysis. Results:There was a significant difference observed between the 3 groups for all the variables except for total number of tubules.Conclusion:BIOMIN–F containing dentifrice was found to produce more completely occluded tubules than dentifrice containing Novamin and Hiora–K on initial application. Number of closed tubules was almost similar in the specimens treated with Novamin and Hiora–K. Novamin containing dentifrice was found to produce more number of partially occluded tubules than BIOMIN–F and Hiora–k.