In the present study, effect of 15% and 17% EDTA using 5.25% NaOCl and 2% Chlorhexidine as irrigant was evaluated on incidence of dentinal microcracks formation during root canal preparation using stereomicroscope.  75 freshly extracted premolars were divided into 1 control, 4 experimental groups (n =15). Teeth in all Groups were coronally flared with GG Drills. Canals were prepared with Protaper File system. In control group only saline used asirrigant, while 2% CHX , 5.25% NaOCl and 17% and 15% EDTA in experimental groups. Crowns were removed 2mm above CEJ using Diamond Discs. Dentinal cracks were evaluated under stereomicroscope 25x.  Chi square Test, Fisher’s Exact Test and Kruskal Wallis, One way ANOVA test were used for Statistical Analysis. It was concluded thatleast number of dentinal microcracks was seen with control group whereas, maximum number of dentinal microcracks with Group 3 in 5.25% NaOCl+ 15% EDTA.