The aim of this research work was to evaluate the level of serum TSH and prolactin in infertile women and healthy age matched controls and to determine the correlation of TSH with Prolactin in infertile women. In this study 100 infertile women and 50 normal fertile women volunteers were selected on OPD basis between age group of 20 to 45 years. They were investigated for serum TSH and Prolactin by Enzyme linked immunosorbent Assay (ELISA) technology. The mean TSH level were 7.92 + 0.78 µIU/ml and 3.82 + 1.71 µIU/ml in the infertile and fertile females (p <0.001). The mean serum prolactin level were 28.4 +6.32 ng/ml and 10.3 + 4.25ng/ml in the infertile and fertile females (p <0.001) respectively. Thus hypothyroidism can be said to be associated with hyperprolactinemia. From our study we have concluded that most frequent hormonal abnormality in female infertility has been hyperprolactinemia, also seen associated with hypothyroidism.