Fungal infections of lungs in tuberculosis patients should be considerd life–threatening infection.500 suspected sputum samples were taken from DOTS centre of TMMC&RC.150 sputum samples was found positive by AFB Z.N. stain. KOH mount was done for the 150 positive samples, in which 38 were positive in direct microscopy. After culture on SDA agar total 46 samples were found positive for fungus, In which 30 were Candida and 16 were aspergillus species. Candida and Aspergillus species identification was done by inoculation on CHROM agar  and by LCB mount respectively. Following Candida and Aspergillus species were found: C.albicans(36.95%), C.tropicalis(15.21%), C.glabrata(8.69%), C.parapsilosis(2.17%), C.krusei(2.17%), A.fumigatus(17.39%), A.niger(10.86%), A.flavus(6.52%). The co–infection of opportunistic fungus with tuberculosis is higher in males than females.C.albicans and A.fumigatus found in high frequency than other species.