Present study was conducted in 200 medical students of Malwa region in which gender determination was done using Face length (FL) and Maximum Facial Breadth (FB). Among all 200 students included in our study, the mean age for males was 19.9±0.114 years (Mean±SEM) and for females it was 20.2±0.096 years. The mean Face length for males was 12.1±0.079 cm and for females it was 10.9±0.04 cm. The mean Maximum Facial Breadth was 12.9±0.065 cm for males while it was 12.4±0.06 cm in females. It is concluded that there was a significant (p<0.0001) statistical difference between males and females for the facial parameters used in this study which shows that these parameters can significantly be used for estimation of gender using the part of skull with intact norma frontalis which allows to measure these parameters during forensic investigations. The FL was a better parameter than FB.