Oral cancer is a sub–type of head and neck cancer, which is any cancerous tissue growth located in the oral cavity. India has highest incidence of OSCC in the world. The development of human cancer is depending on several factors such as DNA damage and DNA repair system. SCGE is used for measuring low level of strand breaks with high sensitivity in cells. The study consists of 115 OSCC patients and 45 control subjects. This study found that, as the severity of OSCC increases, the DNA damage proportionally increases which is marked by MTM. The increased level of MTM significantly in OSCC patient than the healthy controls, indicates that severe DNA damage occurs in OSCC patients during disease progression.