The aim of the study was to determine the correlation of TSH with FSH and LH in infertility in reproductive age group women. In this study 100 infertile women and 50 normal fertile women volunteers were selected on OPD basis between age group 20 to 45 years. They were investigated for serum TSH, FSH and LH by Enzyme linked immunosorbentassay technology. FSH and LH hormonal levels of the infertile women when compared to control groups were significantly found to be lower. The mean±SDof FSH and LH in infertile women were 2.4+ 1.30(p <0.001) and1.32 + 0.21(p <0.001) respectively. Serum TSH concentration was increased in infertile group compared with control group.Thelevel of TSH mean±SD were 7.92 + 0.78(P<0.001) in infertile women.From our study we have concluded that serum TSH was negatively correlated with LH and FSH in infertile groups.