ABSTRACT

IJSR

International Journal of Scientific Research

2277 - 8179

Indian Society for Health and Advanced Research

ijsr-7-5-15533

Original Research Paper

A BACTERIOLOGICAL STUDY OF PRIMARY PYODERMAS IN CHILDREN IN A TERTIARY CARE HOSPITAL

Abarna

Muthulakshmi.M

Dr.

May 2018

7 5 01 02 ABSTRACT

Introduction: Cutaneous bacterial infections may be divided into primary and secondary types. The majority of primary and secondary

pyodermas (cutaneous bacterial infections) are caused by either Staphylococcus aureus or group A Streptococcus. Changing trends are being noted

in the etiological aspect of primary pyodermas and the problem of emergence of drug resistant strains is an ever increasing one. Antibiotic

resistance has become a clinically important consideration in many infections, but methicillin resistant S. auerus (MRSA) which has been a

nosocomial problem for years, is now a common community acquired skin infection.

MATERIALS AND METHODS:

Study Design: Descriptive study. Duration of study: 1 year

Inclusion criteria: Clinically diagnosed cases of primary pyoderma of both sex and age between 1 to 12 years.

Exclusion criteria: Patients with primary pyoderma started on antibiotic therapy.

Sample collection: Smears made from the pus were stained with gramâs stain. Pus was cultured on blood agar and Mackonkeyâs agar and incubated

aerobically at 370 C for 48 hours. Sensitivity of the organisms to antibiotics was tested on Mueller Hinton agar. Antibiotic sensitivity was tested by

disc diffusion technique using Kirby Bauer method.

Observation: Gram staining of the pus demonstrated organisms in 78% cases. Most common organism in gram staining was gram positive cocci.

Culture positivity in this study was 84%. The most common organism isolated in the present study was Staphylococcus aureus accounting for

80.95%, with low percentage of Streptococcus pyogenes (2.38%) and Escherichia coli (2.38%) but with a higher percentage of mixed organisms

(14.29%). The observation of methicillin resistant Staphylococcus aureus was observed in 9.52% of cases. Staphylococcus aureus showed

sensitivity to cloxacillin (100%) followed by first generation cephalosporin (94.11%) and amikacin (73.53%). Streptococcus pyogenes showed

sensitivity to penicillin and gentamicin in all the cases. MRSA was sensitive to amikacin, vancomycin and linezolid in all the cases.

Conclusion : The most common organism isolated in culture was Staphylococcus aureus. MRSA was isolated from 9.52% cases. Staphylococcus

aureus showed sensitivity to cloxacillin (100%) followed by first generation cephalosporin (94.11%) and amikacin (73.53%). MRSA was sensitive

to amikacin, vancomycin and linezolid in all the cases.