Aim:An accurate color reproduction represents the finaljustification level of an estheticrestoration. The aim of this studywas to evaluate the color of permanent maxillary central incisors, lateral incisors and canines, usinga clinical spectrophotometer.Methods: The Vita Easyshade Advance 4.0® intraoral spectrophotometer wasused to determine the color of permanentmaxillary central incisors, lateral incisors and canines of 351 volunteers aging between 18–35 years. The best matches to Vitapan Classical® and 3D–Master® shadeguides were recorded. A one–way analysis of variance and Kruskal–Wallis test wereused to compare L*, a*, b*, c* and h* color coordinates among the 3 types of teeth.Differences between the mean values of all color coordinates were evaluated by use ofBonferroni corrections. Color difference (ΔE*) between 3 types of teethwere calculated from ΔL*, Δa* and Δb* data and the results were compared to ΔE*=3.3acceptability threshold.Results: The most frequently accepted shades were A1(65%) and 1M1(45%) for central incisors, A2(45%) and 1M2(40%)for lateral incisors and B3(40%) and 2M3(45%) for canines.The highest lightness values were found for incisors (85.65 ± 3.2), while the lowest lightnessvalues were found for canines (81.0 ± 3.02). Conclusions: Despite the limitations of this study, color differences amongmaxillary central incisors, lateral incisors and canines were found to be statistically significant, above the clinicalacceptability threshold established. In conclusion, successful esthetic restorationsof permanent teeth of the same patient need an individual approach for color assessment andreproduction of every type of tooth.