IJSR International Journal of Scientific Research 2277 - 8179 Indian Society for Health and Advanced Research ijsr-7-6-15603 Original Research Paper Phenotypic and Molecular Characterization of Extended-Spectrum ?-Lactamase producing clinical isolates of Klebsiella pneumoniae Beri Dr. Malini Shariff Dr. June 2018 7 6 01 02 ABSTRACT

Introduction:K. pneumoniae, often associated with nosocomial infections, has acquired resistance against β–lactam antibiotics. Production of β–lactamase is considered as one of the most common mechanism that accounts for the resistance. It is known to produce significant percentage of ESBLs. Thus, the present study was aimed to characterize ESBL producing isolates of K. pneumoniae.

Material & methods: Sixty strains of K.pneumoniae were collected from patients visiting VPCI. Isolates were subjected to antibiotic susceptibility, phenotypic and genotypic confirmationof ESBL genes.

Results: Isolates exhibited (31–100%) resistance to various antibiotics. 53.33% tested positive using phenotypic confirmatory test. 91.7% isolates were positive for various ESBL genes.The most prevalent ESBL genotypes were blaTEM–1, blaSHV–1 and blaCTX–M–1.

Conclusion: Multiplex PCR is sensitive and rapid method for identifying the ESBL genes. The prevalence of MDR ESBL producing K. pneumoniae appears to be high and majority of them tested positive for TEM–1, SHV–1 and CTX–M–1.