Objectives: The objective of this study is to determine the distribution and antibiotic susceptibility patterns of bacterial strains isolated from patients with urinary tract infections (UTI) at tertiary care hospital in, Rajasthan, as well as identification Multi –drug resistance (MDR) organisms in the population of different uropathogens.

Materials and Methods: Samples receivedby the bacteriology section of microbiology department, from various wards of Hospital will be processed. Standard diagnostic procedures will be followed, using media and stains approved in the laboratory for aerobic culture. Relevant baseline information of the patient will be noted. Internal quality control methods will be observed to ensure correctness of the results. The data thus created will be documented and analysed.

Results: The overall prevalence of UTI in both male and female patients was found to be 65.28%. Among 210 samples, 137 urine samples showed the significant bacterial growth which were comprised of 42 (30.66%) samples from males and 95 (69.34%) from females. Out of the 134 culture isolates , Escherichia coli was the most common 55 (26.19%) followed by Klebsiellapneumoniae (10.95%), Pseudomonas aeruginosa (6.67%), Enterococcus sp.( 5.34%), Staphylococcus aureus (4.76%).The sensitivity was observed as 46.19% to Amikacin, 42.86% to Norfloxacin ,42.38% to Nitrofurantoin ,40.48% to Gentamicin, 30.95% to Meropenem , 25.71% to Ciprofloxacin, , 20.95% to Piperacillin–tazobactam and 19.05% to Linezolid,18.57% to Co–trimoxazole, 17.14% to Amoxicillin–clavulanic acid, 10.4% to and 16.19% to Chloramphenicol and Cefotaxime. On the other hand, as far as the resistance of uropathogens towards antibiotics is concerned, it is found as – 29.05% to Ciprofloxacin, 22.38% to Ampicillin, 21.43% to Amoxicillin–clavulanic acid, 20.47% to Co–trimoxazole, 17.61% to Cefotaxime, 12.86% cefipime and 11.90% to Erythromycin , Pencilin.

Conclusion: Routine monitoring of resistance as well sensitivity patterns is necessary, which will help the clinicians in the empirical treatment of UTI and also for preparation of an antibiotic policy. The high rate of resistance to Ciprofloxacin, Ampicillin, Amoxicillin–clavulanic acid, Co–trimoxazole, Cefuroxime, Cefazolin ,Cefotaxime, of urinary isolates, precludes the use of these commonly used antibiotics for empiric treatment of UTI in India, where as sensitivity towards amikacin, norfloxacin, nitrofurantoin recommends there use for the treatment.