anti–diabetic agent. Therefore, present work was planned to evaluate drug–drug interaction between amiodarone with glipizide. The study also

included evaluation of per se effects of each drug as well as their combinations in alloxan induced diabetic mice.

In this work, we evaluated drug interaction of amiodarone (50 mg/kg p.o.), glipizide (2.5 mg/kg p.o.) and combination of glipizide with amiodarone

2.5 + 50 mg/kg, p.o.). The results showed that glipizide reduced blood glucose levels at 2, 3 and 5 hrs period and the difference were statistically

significant at 3 and 5 hrs in comparison to control group (p< 0.05). The per se showed slight decrease in blood glucose levels which was

comparable to the control group (p>0.05). However, statistically significant difference in comparison to glipizide group was observed at 3 and 5 hrs

(p<0.05) as the blood glucose reduction was significantly less in comparison to that of the glipizide group. It indicated no acute effect of

amiodarone on blood glucose level. However, the combination group (G+A) produces significant increase in blood glucose levels as compared to

control group at 3 and 5 hours and results were comparable to that of the glipizide groups. The results showed significant acute variation on blood

glucose by amiodarone when given in combination with glipizide. So, the finding of DI of glipizide with amiodarone suggest that acute

administration of anti arrhythmic drug did affected blood glucose level as well as if it is used as combination with anti diabetic drug glipizide.