Dyslipidemia is one of the major cause and risk factor for the development and progression of Cardiovascular Disease (CVD), many epidemiological study shows   dyslipidemia is a major cause of morbidity and mortality in bothdeveloped and developing countries like India, Bangladesh , Pakistan and Nepal .Most of the studies have revealed the prevalence of dyslipidemia mostly in general population. The present study attempted to find out the prevalence of dyslipidemia among master health checkup beneficiaries who attended the master health checkup unit of our institution. MATERIAL AND METHODS:The aim of the study was to determine the prevalence of dyslipidemia among master health check–up beneficiaries by retrospective analysis of data from Master health check–up department of IRT– Perundurai Medical College and Hospital, Perundurai, Tamilnadu. RESULT:Among the study subjects, Overall 65.4% were found to have Dyslipidemia which was determined by consideringall four lipid parameters (Total Cholesterol, LDL, HDL and Triglycerides) into consideration.DISCUSSION:The prevalence of dyslipidemia among the study population undergone master health checkup is 65.4%, comparative to study done by Karna.S.K et al(57.7% ) among individuals attending preventive health checkup in rural tertiary care hospital. CONCLUSION:                          The overall prevalence of dyslipidemia in our study is alarming and it highlights the extensive need for screening programs and appropriate intervention programs to reduce the risk factors.