Background: Water pollution including pollution of major rivers have great impact on health of society leading to increased toll of diarrhoeal death. Household water treatment and safe storage (HWTS) interventions can lead to dramatic improvements in drinking water quality and reduction in diarrhoeal diseases. Materials & Methods: A cross sectional study was done to evaluate the MPN (Most Probable Number) count & parasitological estimation of water samples taken from Alaknanda River from Srinagar during the summer month (May–June) of 2016. Both quantitative & qualitative estimation of bacteriological indicator was done using Multiple Tube Method & Medias from HiMedia Laboratories Pvt. Ltd. respectively. Quantitative analysis of MPN count was done by using McCardy’s Tables. Results: MPN count of all the 8 samples was found >150/ml after 48 hour inoculation & qualitative results were also evaluated.