IJSR International Journal of Scientific Research 2277 - 8179 Indian Society for Health and Advanced Research ijsr-7-7-15992 Original Research Paper Urinary lipoarabinomannan (LAM) in the diagnosis of tuberculosis Raj Pandey Dr. Vijay K Shah Dr. July 2018 7 7 01 02 ABSTRACT

 Tuberculosis (TB) is an infectious disease caused by the Mycobacterium tuberculosis. It generally affects the lungs and other parts of the body. A

diagnosis of TB is made by identifying M. TB in clinical samples (e.g., sputum, blood, pus, a tissue biopsy). However, the gold standard culture
process for this slow growing organism can take 2–6 weeks for result. The detection of lipoarabinomannan (LAM), a 17.5–kDa glycolipid
component of the mycobacterial cell wall, can be better approach to diagnose active TB. LAM is released from metabolically active & degrading
mycobacterial cell wall and is detectable in urine. Detection of mycobacterial antigens in urine has several potential advantages compared with all
currently used diagnostic method. The main aim of present study is to diagnose TB by detecting urinary LAM.
In this study we have taken microbiologically confirmed TB patients and healthy individual as control. 5 ml random urine sample was collected
from Pulmonary TB patients, Extrapulmonary TB patient and normal healthy individual in sterile containers and quantitative estimation was done
by ELISA. Urinary LAM of pulmonary TB (322.24 ± 195.73) pg/ml is high as compare to the Extrapulmonary TB (245.95 ± 217.92) pg/ml. LAM
value of Pulmonary TB and Extarpulmonary TB as compared with healthy individual (control) (41.06 ± 9.77) shows statistically highly significant
(p<0.0001). Urinary LAM can be used as a marker for the diagnosis of tuberculosis.