Alpha–tocopherol is the subspecies of vitamin–E antioxidant vital for maintaining cell membrane integrity.Selenium is the cofactor of glutathione peroxidase which detoxifies hydrogen peroxide and other organic peroxides. Alfa–tocopherol and Selenium demonstrate accelerated wound healing. However, little is known about its effect on periodontal tissues. Hence the present study was an attempt to determine the effect of alpha–tocopherol, selenium and alpha tocopherol–selenium combination on periodontal ligament fibroblast cell proliferation and migration. Primary cultures of periodontal ligament fibroblasts cells were treated with the test group compounds alpha tocopherol, selenium and combination of alpha tocopherol and selenium. Migration of periodontal ligament fibroblasts cells was assessed by in vitro scratch test whereas proliferation was determined by the MTT assay. The results of the study indicated that of the three test groups (α–tocopherol, selenium and combination) used for detecting the periodontal ligament fibroblast cell migration and proliferation, combination of α–tocopherol, selenium demonstrated maximum effect.