females. The classic and short segment Hirschsprung disease is the most common type of Hirschsprung disease and the rectosigmoid colon is the

most common site for this disease , the patients most commonly present with clinical manifestation of intestinal obstruction such as delayed

passage of meconium, constipation etc. Though histopathological examination is the gold standard , Calretinin is reliable in detecting the presence

or absence of ganglion cells . It has many advantages that it can be carried out in paraffin embedded tissue sections. Also binary pattern of

interpretation; negative indicates a diagnosis of Hirschprung disease and positive rules out the diagnosis of Hirschsprung disease. Thus calretinin

can be a valuable cost effective diagnostic aid for diagnosis of Hirschsprung disease.