Polycystic ovary syndrome (PCOS) is a heterogeneous disorder characterized by hormonal imbalance,which in turn results in infertility in young women. Traditionally, along with ultrasound reports, changes in luteinizing hormone (LH), follicle stimulating hormone (FSH), and the LH/FSH ratio are employed for the diagnosis of PCOS. In the present study, we report the usefulness of the serum Anti–Müllerian hormone (AMH) levels in PCOS diagnosis. The study group had 125 PCOS cases andan equal number of healthy controls. Their serum status of Estradiol (E2), LH, FSH, AMH, and prolactin (PRL) were estimated by Electro–Chemiluminescence immunoassay (ECLIA). We observeda significant rise inLH, FSH, LH/FSH ratio and AMH in PCOS cases when compared to control subjects, whereas no significant variation was observed in estradioland PRL. The LH/FSH ratio was inconclusive in 11 of the 125 PCOS cases (8.8%), where AMH was found to be elevated in all of them. Hence, we recommend the elevated AMH as a potential indicator for the diagnosis of PCOS.