Dermatoglyphics has been accepted as a simple and cost–effective method for determining various chromosomal,medical disorders and as well as hemoglobinopathies.β–thalassemia is a common disorder of hemoglobin synthesis in India. Keeping this in view, thepresent study was planned to study the association of ‘atd’ angles with β–thalassemia major cases in comparison to controls. A case control study was carried out in 400 study participants (200 cases & 200 controls) in department of Anatomy, R.N.T. Medical College & attached hospitals, Udaipur, Rajasthan. ‘atd’ angle of all study participants was measured. It was concluded that there was significant association of ‘atd’ angle found with β– thalassemia major. This dermatoglyphic parameter can be used as a diagnostic tool for the screening of β–thalassemia major.