Introduction :–Acinetobacterspecies have be­come the most common etiology of hospital acquired infections due to the present frequent use of wide spec­trum antibiotics.¹Infections due to resistant Acineto­bacterbaumanniiisolates cause difficulties in treatment.

Objective :–current study was to determine the susceptibil­ity rate of different Acinetobacterspecies, isolated from various clinical samples of patients.

Methods :–43 strains of Acinetobacterspecies were isolated from different clinical specimens and fully characterized by standard procedures with  Antimicrobial susceptibility patterns as per guidelines of CLSI.

Results: Majority of isolates of Acinetobacterspecies 22 (51.16%) wereobtained from specimens of pus. The isolated pathogens showed 100% sensitive to Polymyxin –B and Colistin.

Conclusion: The present studied showed that aminoglycoside,quinolone and carbapenem resistant Acinetobacter species is increasing gradually.