In developing countries, exposure to potential road traffic injury has increased largely. The present study was undertaken to assess knowledge of teenagers regarding prevention of road traffic accidentat RAHS School. In order to achieve the objectives of the study a descriptive research design was used. Fifty students who were studying in class 11^th and 12^th were selected by simple random sampling technique. Their knowledge regarding road traffic accident was assessed by self structured knowledge questionnaire which comprised of two parts. Part I–socio demographic profile of students and Part –II structured knowledge questionnaire on road traffic accident. Findings revealed that (72%) students had below average knowledge followed by 20% had average knowledge and only 8% had good knowledge regarding road traffic accident. Chi square test showed that there was no significant association between level of knowledge with socio demographic variables. The findings revealed that majority of students had below average knowledge, hence it is recommended that education should be given on preventive measures of road traffic accidents by teachers and health professionals.