Aim: Toisolate, identify and characterize the prevalence of non– fermenting gram negative bacteria (NFGNB) along with their antimicrobial resistance pattern among the patients attending a tertiary care Hospital in Meerut.Materials and Methods: The isolates of NFGNB obtained from various samples were identified by standard bacteriological technique andVITEK® 2 system (Biomerieux , France) . Antimicrobial susceptibility   test wascarried out to assess the resistance profile both by Kirby Baeur disk diffusion method and VITEK® 2 system (Biomerieux , France) .Result:A total of 16,296 clinical samples from the indoorand outdoor patients were processed in the bacteriology laboratory. Isolation rate of non–fermenters was 7.82% (1274/16296).Pseudomonas spp.was the predominant isolate (66.56%)followed by Acinetobacterspp.(27.16%). Other isolated non–fermenters were Burkholderiacepaciacomplex(BCC)(1.57%),Stenotrophomonasmaltophila(0.86%), Sphingomonaspaucimobilis(0.71%),Achromobacterxylosoxidans(0.16%),Ochrobactrumanthropic(0.16%).Conclusion:NFGNB are emerging as important opportunistic pathogens and are mostly resistant to commonly used antimicrobials. Therefore early diagnosis and initiation of appropriate antibiotic therapy would help in efficient management of patients and result in reduction of morbidity and mortalitycaused by these multi drug resistant organisms.