As a matter of fact various antibiotics are present nowadays for wound management but more than 80% of the world’s population especially developing countries still depend upon herbal medicine for this purpose. This may be because of resistance development in pathogen against antibiotics. Moreover, safe, effective, easily accessible and eco–friendly herbal medicine compel for its worldwide uses. Among these, Azadirachta Indica (neem) is the most useful traditional plant having potent anti–inflammatory, analgesic,antipyretic,antioxidant andantimicrobial properties which helps in accelerating the process of wound healing and is being used for this purpose from centuries. This study was conducted on thirty guinea pigs grouped randomly into three groups and following standard surgical procedures with the help of a sterile Bard Parker blade two incised wound of 2 cm long and 0.5 cm depth were made on opposite thigh muscle making a total of two wounds per animal.Control group was treated with Vaseline, treatment group with aqueous extract of “Azadirachta Indica” leaves and a reference standard had been treated with topical application of framycetin sulphate (1% w/w)twice daily for seventeen days.Estimation of wound healing was done by shrinkage of wound size on days 3, 5, 7, 9, 11, 13 and 17 by randomly selecting 10 guinea pigs on same site.  The data were analyzed by applying unpaired t–test. On the basis of the outcome of the  present study it has been observed that aqueous extract of Azadirachta Indica leaves are highly effective in accelerating wound healing  process as compared to control(Vaseline treated).However, a further study is warranted in human.