The major antigen of Rh blood group system is the Rh D antigen.. The “weak D” actually refers to red cells with the aberrant Rh–D protein expressing reduced membrane surface D antigen.This study was conducted in the Blood Bank of Rajendra Institute of Medical Sciences, Ranchi, Jharkhand. It is a retrospective study and data of 3yrs from January, 2015 to December, 2017 was collected.A total of  1,66,000 healthy blood donors were tested out of which total of 2013 (1.21%)  blood donors turned out to be Rh–D negative during a span of 36 months from January, 2015 to December, 2017 and on further testing 7 (0.35%) of them turned out to be weak D positive.For safe blood transfusion & to prevent transfusion related complications, comprehensive national transfusion guidelines need to be laid down to standardize the protocol for D antigen testing for donors as well as patients.