Acute heart failure pertains to the symptom and signs the sudden onset of heart failure, or worse, which requires urgent medical treatment and hospitalization. It is a severe life–threatening disease, and it is the most common in clinic. Due to its clinical onset, illness, rapid disease progression, and prognosis, there is a great uncertainty due to the high expectations of patients. At the same time, medical disputes in the medical diagnosis and treatment of this disease can easily occur. Hence, there is a need to perform a correct diagnosis and evaluate this in a short period of time, in order to allow for reasonable treatment measures and improve the prognosis of patients. It is difficult to diagnose heart failure, while echocardiography can effectively evaluate cardiac systolic function. However, diastolic function evaluation is limited and cannot be applied for emergency situations. Hence, there is a need to seek a more convenient and feasible method with high specificity and sensitivity. Plasma brain natriuretic peptide (BNP) and beta–endorphin (β–EP) are important markers for heart failure. Therefore, β–EP and BNP levels have been monitored for the early diagnosis and treatment of acute left heart failure. The details are reported as follows.This study aims to evaluate the diagnostic value of beta–endorphin (β–EP) and brain natriuretic peptid (BNP) plasma concentrations for the early diagnosis of acute left heart failure and atrial fibrillation.A total of 40 patients were included. These patients comprised 22 male and 18 female patients,and 20 healthy subjects who underwent physical examinations in the Outpatient Department during the same periodwere included and assigned to the control group.Enzyme–linked immunosorbent assay was performed to detect the plasma concentration of β–EP and BNP in the treatment and control groups, and electrocardiogram targeting was performed to determine the left ventricular ejection fraction (LVEF).BNP, β–EP, and LVEF levels were higher in the treatment group compared with the control group. The P–values were .015, .019, and .026, respectively, which were <.05. The difference was statistically significant.In conclusion, β–EP and BNP have high specificity and sensitivity for identifying early intense left heart failure and atrial fibrillation in patients, which is advantageous, simple to perform, and appropriate for clinical applications.