Asymptomatic bacteriuria (ASB) is Urinary Tract Infection without symptoms, which is associated with significant maternal and foetal risks. The study aimed to find, incidence of ASB in pregnant women at Nair Hospital, Mumbai. Study included 3000 pregnant and 300 non–pregnant women. Collection, transportation, and microscopic examination of urine and identification of isolates were carried out by standard methods. In pregnant women, majority ASB cases (10.0%) were in age group of 36 to 40 years, while least (7.67%) were in age group of 30–35% years with mixed infection of two pathogens. In non–pregnant women mixed infection was absent.Prevalence of ASB was twice more common in pregnant women than non–pregnant women.(p value< 0.0001). In both groups,E.coli was the predominant isolate. The results showed an association of ASB with age and appeared multifactorial. Hence, routine screening for ASB in pregnant women would allow early treatment to avoid complications.