Aims and objectives : Study  of  effect of Problem based learning as innovative teaching learning methodology   to  first  year M.B.B.S  and its  relation to its  effect on University Examination Results  in Biochemistry of   first year   students .Material and Methods :This prospective study was  undertaken  in Department of Biochemistry ,MGM’S Medical college ,Aurangabad.Total 1350 students from last 10 batches from 2008 to 2017 were taken for study.We studied average of passing percentage of  batches of First M.B.B.S. students in last 10 years and studied the effect of Problem based learning  conducted in Biochemistry for  first  year M.B.B.S students  and its  relation to  University Examination Results.Observations and Results :In our study  we compared  results of  the batches  in whom students were exposed to problem  based learning  as innovative  teaching learning methodology  in Biochemistry with  the  earlier batches in which  the  innovative  method was not notused.The  mean average  result of batches in whom innovative innovative  method PBL was used was   91.2 ±8.8 in comparison to 77.6 ± 3.07  in the batches in whom PBL was not used .The difference is statistically significant. Conclusion: This shows that inclusion of PBL as innovative teaching learning  methodology in Biochemistry to First M.B.B.S. students   helps in better understanding of clinicobiochemical correlation of  topics and effectively dispensing of teaching learning process including emphasis on student centric methods such as problem based learning and outcome of the students passing rate of university examination is consistently good. This has been shown  by average passing rate above 80 % consistently  from last 04 years university results  which  is  definitely correlating to  innovative  teaching learning method used  in Biochemistry of first M.B.B.S.and its successful implementation by Department of Biochemistry,MGM Medical college,Aurangabad.