Background:  Measurement of bone marrow iron (hemosiderin) by histochemical Prussian blue/Perl’s staining is taken as gold standard to diagnoseiron status/iron overload and to differentiate morphologically similar anemia due to chronic disorder versus nutritional causes.

Aims:  To evaluate dynamics of iron metabolism in various conditions.

Study design: This prospective study was performed in cases who have undergone bone marrow aspiration to access the bone marrow iron stores

Subjects & methods: To correlate serum levels of ferritin, iron, total iron binding capacity with morphological bone marrow aspiration iron Gale’s grading in 100 consecutivecases.

Statistical Analysis Used: Chi–square and Spearman correlation tests were done using SPSS 18.0 software (IBM Inc).

Results: Statistical data proved that P value of BM iron grading is < 0.001 with both serum ferritin and TIBC, however overall correlating best with serum ferritin (Spearman correlation 0.865) as compared to TIBC and serum iron.