Aim: To assess the marital status differences in the level of knowledge, attitude and practices related to HIV/AIDS in patients and their relatives coming to Saoner Rural Hospital as well as primary health centers and sub–centers under Saoner Rural Hospital. Materials and Methods: The cross sectional study was performed through a self–administered questionnaire in local Marathi dialect to 500 people between 20–30 years of age. SAS version of 9.1.3 was used for descriptive statistical analysis. Results: There was a significant difference (p value <0.0001) in the levels of knowledge among singles vs. married where singles were better knowledgeable compared to married. This put married at high risk for practices in contrast to singles (p value 0.0002). However married demonstrated statistically significant positive attitude against singles (p value <0.0001). The people who did not ask doctor to use disposable needle and syringes differ significantly (p value 0.0003) in marital status. Conclusion: Marital Status is an important factor for Knowledge, Attitude and Practices related to HIV/ AIDS in Rural Area.