ABSTRACT

IJSR

International Journal of Scientific Research

2277 - 8179

Indian Society for Health and Advanced Research

ijsr-7-10-16870

Original Research Paper

IN VITRO EVALUATION OF ANTIOXIDANT ACTIVITY OF TENELIGLIPTIN BY FRAP AND NITRIC OXIDE ASSAY

Ravichandar

Dr. Dr.

Akila L

Dr.

October 2018

7 10 01 02 ABSTRACT

The aim of the study is to evaluate the antioxidant activity of the newer anti–diabetic drug by using FRAP and nitric oxide assay. Methodology: FRAP assay was done by using Benzie and Strain method (1999).The antioxidant activity is measured by the reduction of ferric complex (Fe3+ tripyridyltriazine) which is colourless

to blue–colored ferrous complex (Fe2+ tripyridyltriazine) at 593nm.This action is due to the electron donating antioxidants at lowpH.FRAP values are calculated by comparing the absorbance change at 593 nm in test reaction mixtures with that of the standard (ascorbic acid).Nitric oxide assay was done by using Kumar S et al. method (2008).In this method Sodium nitroprusside in aqueous solution at physiological pH spontaneously generates Nitrite oxide which interacts with oxygen to produce Nitrite ions, which can be measured at 550nm by spectrophotometer in the presence of Griess reagent and was compared with that of the standard (ascorbic acid).Since Teneligliptin which is the novel DPP4 inhibitor plays a vital role in the management of type 2 diabetes mellitus we have evaluated the antioxidant potential of this drug by using FRAP and nitric oxide assay.